Optical High Resolution Episcopic Microscope
cutting/sectioning imaging system
There is now an instrument for the Optical HREM technique in your laboratory. Available to the UK, USA and Europe.
This technique achieves imaging for 3D reconstruction purposes. For whole samples/embryos by multiple sectioning (accurate cutting) samples of sizes 1mm to 20mm. High resolution imaging of each cut surface thus lends itself to 3D reconstruction of the whole sample and accurate measurement. OHREM is an episcopic imaging procedure that uses plastic rather than wax as an embedding medium. Samples are embedded in a resin that has been made highly fluorescent by the addition of dyes. The sample block is mounted in the system and a section is cut from the top of the block.
Opaque and translucent tissue is imaged by its ability to suppress the fluorescence of the plastic. This results in excellent quality, high resolution images of the block surface, irrespective of the tissue type or developmental stage. A standard section thickness of 1-5 micron is achievable and no re-alignment of the sections is required. Sample dimensions can be from a few mm's to approximately 20mm maximum overal samples size.
Different tissue types are easily distinguished and as no realignment of the sections is necessary, the sections are simply imported directly into whatever 2D or 3D visualisation software you may be using.
OHREM is suitable for use over a broad range of magnifications.
Many examples of HREM images and further information may be found on the DMDD web site. Click on the 'Our Research' tab to explore.
DMDD - Deciphering the Mechanisms of Developmental Disorders
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