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 Ohrem Embryo and small organ 2D and 3D sectioning/imaging system

Imaging the Block Face using Fluorescence

This technique achieves Imaging for 3D reconstruction purposes and others. It has the highest resolution and best image quality of any system available and is used extensively on whole mouse embryos, embryonic organs and many different tissues types. OHREM is an optical based system and so very flexible in its imaging possibilities and can be configured for multiple fluorescent channels. It is used in laboratories world wide for 3D imaging of whole samples/embryos by multiple sectioning from 1 micron to 10 microns section sizes.


 

High resolution images of the block surface are taken following each cut and the fully aligned sections can be used directly to 3D reconstruct the whole sample or accuratly measure. 

HREM is an episcopic imaging procedure that uses plastic rather than wax as an embedding medium, the samples are embedded in the resin, that has been made highly fluorescent by the addition of dyes, so opaque and translucent tissue is imaged by it suppressing the fluorescence. This results in excellent quality, high resolution images of the block surface, irrespective of the tissue type or developmental stage - different tissue types are easily distinguishable. A standard section thickness of 1-10 microns is achievable and the sections may simply be imported directly into whichever 2D or 3D visualisation software you wish to use.

OHREM is suitable for use over a broad range of magnifications.

Many examples of HREM images and further information may be found amongst other places, on the DMDD web site - click  the link DMDD below then goto the 'Our Research' tab to explore sample preparation and much more.







Current HREM Clients



3           Francis Crick Institute            
  Deciphering the Mechanisms of Developmental Disorders (DMDD)    
  London , England              
  https://www.crick.ac.uk/            
  https://dmdd.org.uk/            
                   
1 Agricultural Research Organization - the Volcani Center    
  Israel Institute of Animal Science          
  Rishon LeZion, Israel            
  http://www.agri.gov.il/en/units/institutes/3.aspx        
                   
1 Institute-Pasteur                
  Heart Morphogenesis            
  Paris, France              
  https://research.pasteur.fr/en/team/heart-morphogenesis/      
                   
1 Regeneron Pharmaceuticals          
  Tarrytown, New York            
  https://www.regeneron.com/            
                   
1 Laboratory of Artificial & Natural Evolution (LANE)      
  Dept of Genetics & Evolution | University of Geneva      
  Geneva, Switzerland            
  https://www.lanevol.org/            
                   
1 UCL Centre for Advanced Biomedical Imaging (CABI)    
  London, England              
  http://www.ucl.ac.uk/cabi            
                   
1 University of Vienna              
  Center for Anatomy & Cell Biology          
  Vienna, Austria              
  https://anatomieundzellbiologie.meduniwien.ac.at/?L=1      
                   
1 Department of Physiology Anatomy & Genetics            
  Le Gros Clark Building, S Parks Rd          
  Oxford, England              
  https://www.dpag.ox.ac.uk/