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High Resolution Episcopic Microscopy(HREM) instrument with Heart background

High-Resolution Episcopic Microscopy produces 3D datasets from detailed 2D sections. This 3D microscopy technique gives volumetric datasets on a wide range of samples.

  • Image 1-6 micron complete voxel dimensions.

  • Capable of capturing dense tissue and bone at high contrast.

  • No sample clearing, optical sectioning or interpolation.

  • Complete 3D imaging system inclusive of specific software, instrument and other hardware. 

High Resolution Episcopic Microscopy(HREM) Cross Sectional Image for 3D data

What is High Resolution Episcopic Microscopy (HREM)?

High Resolution Episcopic Microscopy (HREM) is a 3D imaging technique for visualising samples ranging from 1-30mm. Resin mounted samples are stained, then sectioned repeatedly with the remaining block face imaged, creating high-resolution stacks at 1–8-micron voxel resolution.

Advantages of High Resolution Episcopic Microscopy (HREM)

Flexibility for a Range of Samples

High-Resolution Episcopic Microscopy (HREM) delivers exceptional clarity and resolution, even when imaging denser tissues or tissues with varying opacities.


Unlike conventional techniques, High Resolution Episcopic Microscopy (HREM) eliminates the need for tissue clearing, making the imaging process more efficient and convenient. HREM produces high-contrast results, enhancing the visualization of anatomical structures and details.

  • Capable of visualising samples up to 30mm

  • Suitable for bone and denser tissue samples

  • High contrast images 

High Resolution Episcopic Microscopy(HREM)
High-Resolution Episcopic Microscopy Cross Section

High Resolution for All Tissue Types

High-Resolution Episcopic Microscopy (HREM) offers a variety of resolutions with scanning stages and a choice of optics. It can capture single shots or high-resolution scans at 100x magnification with blending, no blocking.


With sections as thin as 1 µm, from our speciality section system, HREM achieves voxel resolutions of 1-8 µm³, enabling the identification of fine details like individual nerves and blood vessels, surpassing other techniques.

  • 1-8 micron voxel size 

  • 100x magnification 

  • XY scanning stage integrated into the software available for larger samples 

High-Resolution Episcopic Microscopy Mouse Embryo

HREM Easy Data

High Resolution Episcopic Microscopy (HREM) data is readily applicable for both 2D and 3D analysis. Simple software packages like Fiji and Dragonfly can be used to generate impressive images by inverting the LUT (Lookup Table). 


High-Resolution Episcopic Microscopy (HREM) images can be manually segmented, owing to their user-friendly 2-Dimensional format, enabling precise measurement of specific features.

  • Data is stored as image files for easy manipulation 

  • Stored image sizes for easy workflow 

  • No custom image analysis software required 

Applications for HREM

High Resolution Episcopic Microscopy (HREM) is used in the study of mouse organs (such as brains and hearts) whole mouse embryos or even plantsAs the field of HREM continues to expand, new research papers emerge each year, showcasing the potential in different applications.


Integrate High-Resolution Episcopic Microscopy (HREM) into your research, gaining a valuable tool in your workflow. Explore the accomplishments of our satisfied HREM customers by delving into their published papers.