Cleaning an Objective

Microscope objectives are a vital component to a microscope but often can be left unclean, which can lead to poor image quality or even damage. This guide demonstrates how to clean an objective so that you can improve your image quality and prevent any damage.

It is always worth consulting with documentation/manufacturer before following this guide.

When to perform an objective clean?

• Lab use - once a week/on occurrence of contamination

• Oil immersion - after each use

• Occasional use - monthly/as needed

Cleaning Procedure

Materials needed

1. Lens tissue/lint free swab (we recommend lens tissue for larger lenses and a swab for smaller lenses).

2. Gloves

3. Blower (Not compressed air)

4. Optical grade cleaner (100% ethanol, isopropanol, commercial lens cleaner)

5. Optional - Immersion oil remover, such as Xylene

Cleaning Procedure

1. Put on gloves, this will ensure no finger marks are left on the objective.

2. Use an air blower to remove loose dust and dirt, it is best to use blow at an angle to the surface of the objective lens.

3. Solvent cleaning

   1. Fold lens tissue/prepare lint free swab

   2. Moisten the lens tissue/swab with the cleaner. Do not dampen.

   3. If cleaning an oil objective use xylene to remove the oil from the surface.

   4. Gently drag the tissue/swab in a circular motion starting at the centre of the optic and working the way out of the optic.

4. Use a fresh piece of lens tissue/swab to dry the objective as it can leave residue.