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Optical High Resolution Episcopic Microscopy(HREM) System

High Resolution Episcopic Microscopy(HREM) is a 3D destructive imaging technique comprising of 2D sections for structural analysis of samples.

Benefits of the HREM imaging technique:

  • Produces 1-8 micron complete voxel resolution over an entire volumetric dataset

  • Image dense tissue and bone at high contrast without variation

  • No sample clearing, sample clearing or interpolation

  • Capable of imaging up to 60mm samples


HREM as a 3D imaging technique of ex-vivo samples gives multiple benefits over other techniques such as resolution cost and more.

HREM Mouse Section


Image a wide range of samples and density’s with Optical HREM instruments. Image varying density’s and tissue types, including plant matter, with surface based imaging.

Easy Data

Optical HREM provides straight forward images as an output with no processing. Captured in 2D stacks easily converted into 3D volume renderings.

3D Imaging HREM Resolution


Output of 1-8 micron voxel resolution gives the flexibility to visualise detail unseen in other techniques and systems. 

HREM Mouse Embryo Image


High-resolution episcopic microscopy (HREM) as a technique requires no sample clearing or interpolation. This opens up the floor to 3D imaging samples in detail that previously could not be imaged in such detail.

What is High-Resolution Episcopic Microscopy(HREM)?

High-Resolution Episcopic Microscopy (HREM) is a 3D imaging technique for visualising samples ranging from 1-30mm. Resin mounted samples are stained, then sectioned repeatedly with the remaining block face imaged, creating high-resolution stacks at 1–8-micron voxel resolution.

We offer two systems for performing HREM, the Micro and Ultra. As well as other products.

Mouse Heart 3D Imaged with HREM

Flexibility for a Range of Samples

High-Resolution Episcopic Microscopy(HREM) delivers exceptional clarity and resolution, even when imaging denser tissues or tissues with varying opacities.


Unlike conventional techniques, eliminate the need for tissue clearing and interpolation, making the imaging process more efficient and convenient. HREM produces high-contrast results, enhancing the visualization of anatomical structures and details.

  • Capable of visualising samples up to 30mm in one capture or greater with scanning

  • Suitable for bone and denser tissue samples

  • High contrast images even in higher density areas

Agricultural Morphology Imaged with HREM

High Resolution for All Tissue and Sample Types

High-Resolution Episcopic Microscopy(HREM) image of mouse embryo section.

High-Resolution Episcopic Microscopy (HREM) offers a variety of resolutions with scanning stages and a choice of optics. It can capture single shots or high-resolution scans at 100x magnification with blending, no blocking.


With sections as thin as 1 µm, from our speciality section system, HREM achieves voxel resolutions of 1-8 µm³, enabling the identification of fine details like individual nerves and blood vessels, surpassing other techniques.

  • 1-8 micron voxel size resolution

  • 100x magnification

  • XY scanning stage integrated into the software available for larger samples

Easy Data with HREM

High Resolution Episcopic Microscopy(HREM) image of a 3D mouse embryo.

High-Resolution Episcopic Microscopy (HREM) data is readily applicable for both 2D and 3D analysis. Simple software packages like Fiji and Dragonfly can be used to generate impressive images by inverting the LUT (Lookup Table). 


HREM images can be manually or automatically segmented in the user-friendly 2-D image format, enabling precise measurements.

  • Data is stored as image files for easy manipulation (jpeg,tif and more)

  • Stored image sizes for easy workflow

  • No custom image analysis software required

Applications for HREM

High Resolution Episcopic Microscopy(HREM) is used in the study of:

As the field of HREM continues to expand, new research papers emerge each year, showcasing the potential in different applications.


Integrate into your research, gaining a valuable tool in your workflow. Explore the accomplishments of our satisfied HREM customers by delving into their published papers.

HREM Mouse Embryo Heart


"HREM data has allowed the DMDD team to identify nearly 400 different phenotypes in knockout mouse embryos. These range from large-scale organ malformations to the abnormal development of nerves and blood vessels."

Tim Mohan

Product Options

View product options for HREM 3D imaging system such as:

  • Micro Optical HREM system for lower budget high quality 3Ds up to 25mm.

  • Ultra Optical HREM systems designed for a range of images

  • Consumables for HREM

3D Imaging System HREM Ultra

Contact our High-Resolution Episcopic Microscopy (HREM) Experts

Want to know more about HREM, ask for a quote or get questions answered. Contact us and we can help answer all your questions.


Thank You!

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