What makes HREM a good tool for heart imaging in small models?

HREM provides high resolution and 3D imaging capabilities, allowing researchers to visualize the complex anatomy of the heart in great detail and measure in 3 dimensions.

Can HREM be used for imaging hearts at different developmental stages?

Yes, HREM is highly effective for imaging hearts at various stages of development, particularly in embryos and pups.

Can HREM be used to image other species and organs?

Yes, HREM can be used to image many different sample types and density’s such as zebrafish.

High-Resolution Episcopic Microscopy / Applications / Heart Imaging

3D Heart Imaging in Mouse and Embryos Using HREM

3D Imaging of Mouse Cardiovascular Systems at Micron Resolution

High-Resolution Episcopic Microscopy (HREM) provides detailed 3D imaging of heart structures in mouse, mouse embryos, and chicken embryos, advancing cardiovascular research

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Mouse Heart in 3D Imaged with High-Resolution Episcopic Microscopy (HREM)

Mouse Heart 3D Credit: Dr. Aseel Abbad (University of Nottingham, UK)

Imaging Heart Development in Mouse and Embryo Models

High-Resolution Episcopic Microscopy (HREM) provides precise 3D datasets of cardiac structures in mouse and chicken embryos, with sample sizes ranging under 1mm up to 25mm.

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3D Heart Imaging in Small Models with HREM

High-Resolution Episcopic Microscopy (HREM) is a 3D histology imaging technique that gives insights into the cardiac structures of small models such as mice and embryos, producing detailed 3D mouse heart imaging and cardiac morphology analysis. Whether you're studying the developing hearts of mice embryos (embryonic mouse heart development, E9.5–E14.5 cardiac morphogenesis) or chicken embryos, HREM delivers the clarity and detail required to advance your cardiovascular research, preclinical cardiac imaging, and congenital heart defect (CHD) studies.

By producing high-resolution, 3D reconstructions of the heart in different species, supporting cardiac phenotyping, gene knockout heart phenotype analysis, and structural heart disease model research.

3D reconstructed image of a mouse embryo heart captured using High-Resolution Episcopic Microscopy (HREM)

How High-Resolution Episcopic Microscopy Enhances 3D Heart Imaging

High-Throughput Imaging

HREM allows for detailed 3D mouse heart imaging in quantity ideal for knockout trials or high throughput, repeatable imaging applications. With possibilities for multiple samples per run.

Detailed Visualization

HREM technique provides a clear view of the intricate structures of the heart, including valves, chambers, and vessels for further study.

Quantitative Analysis

HREM enables precise measurement in 3D which enhances Heart Imaging and analysis of heart structures, which is critical for understanding heart development and disease.

Benefits of HREM for Embryonic Heart Imaging

HREM offers a range of benefits for imaging embryonic and pup hearts, supporting cardiac morphology analysis and embryonic mouse heart development

Optical Resolution
- HREM offers resolutions to 1um in the X/Y/Z planes which allow for visualising vessels and larger structures in context
Density
- Denser tissue can be imaged which allows for improved clarity of internal structures along with bone structures, supporting high-resolution tissue morphology analysis
Repeatability
- Without the need for clearing HREM offers a repeatable method for measuring in the 3D context
Contrast
- HREM images provide a clear contrast images, especially for sample sizes of hearts e.g below 1mm up to 20mm

3D Embryonic mouse structure with plane views

Applications of HREM in Cardiac Research

HREM allows researchers study heart development and disease in animal models.

Heart Development in Mice
- HREM enables detailed 3D imaging of heart development in mice, offering critical insights into congenital heart.
Mice Embryo Cardiac Imaging
- Imaging of cardiac imaging the full embryo, making it possible to study the heart at various stages of embryonic development (E9.5–E14.5).
Chicken Embryo Heart Imaging
- Effective for imaging chicken embryos, providing valuable data for comparative studies in cardiac research.
Larger Samples
- HREM can be used to image denser larger cardiac systems, such as pups, supporting small animal heart imaging and 3D tissue morphology analysis.