High-Resolution Episcopic Microscopy / Applications / Mice and Zebrafish
3D Imaging of Mice and Zebrafish with High-Resolution Episcopic Microscopy
Imaging of Mice and Zebrafish in 3D at Micron Scale
Image whole 2D stacks of micron scale images, aligned for 3D morphological reconstruction using High-Resolution Episcopic Microscopy (HREM).
Imaging Mouse and Zebrafish Samples in 3D Overview
High Resolution Episcopic Microscopy (HREM) is used to capture mouse and zebrafish samples, from whole organs to whole samples. OHREM instruments produce large high quality, high throughput stacks with no need for sample clearing or distortion to the block.
This microscopy technique allows for imaging both zebrafish and mice in full 3D, combined with whole 2D images for analysis.
Applications in Developmental Biology and Phenotyping
HREM can applied across a wide range of studies in mouse and zebrafish models, including:
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Knockout and phenotyping trials - Imaging whole embryos and pups as part of large-scale screening and developmental biology analysis workflows.
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Cardiovascular imaging - Visualise whole hearts in both 2D sections and fully constructed 3D models.
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Neuroanatomy and brain imaging- Image whole brains for detailed analysis of tissue architecture and structural organisation.
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Whole organ morphology - Imaging individual organs such as kidney and other tissues for anatomical and developmental studies.
Why Use HREM for Mouse and Zebrafish Imaging
Larger sample sizes
Sample sizes from under 1mm up to 25mm, more if requested allowing for imaging a whole range of mice and zebrafish samples.
Fine structural detection
Enables identification of small anatomical changes relevant to phenotyping and disease studies.
High resolution
HREM technique provides 1–6-micron voxel size resolution, which allows for detail beyond other 3D technology.
Aligned image stacks
Serial block-face imaging produces inherently aligned datasets, reducing the need for complex registration and improving accuracy in 3D reconstruction.
No sample clearing
No sample clearing, HREM does not require clearing of samples for the images to be produced.
Excellent soft tissue contrast
Histology-like contrast enables clear visualisation of internal structures such as organs, vasculature, and fine tissue boundaries.
3D Mouse Embryo and Mouse Pups in Full
Optical HREM systems are widely used to capture full morphological data in 3D of mouse embryo and mouse pup samples. High-Resolution Episcopic Microscopy provides high-contrast imaging with fine structural detail throughout the specimen.
Imaging Mouse Embryos, Organs, and Whole Specimens
High-Resolution Episcopic Microscopy is widely used to image individual organs from mouse embryos and pups. Optical HREM systems are commonly applied to capture detailed datasets of organs such as the heart, kidney and other tissues for structural developmental analysis.
Zebrafish Imaging in 3D
Samples such as zebrafish/catfish/chick embryos can be used, without the need for clearing. HREM has the advantage of imaging such samples with denser tissue and bone.
Why Choose HREM Over Other Imaging Techniques
Researchers often evaluate multiple imaging methods when studying morphology or development. High-Resolution Episcopic Microscopy is particularly well suited to applications requiring high-resolution structural detail, consistent contrast, and accurate 3D reconstruction of small biological specimens.
Zebrafish Imaging in 3D
Samples such as zebrafish/catfish/chick embryos can be used, without the need for clearing. HREM has the advantage of imaging such samples with denser tissue and bone.
