Mouse Heart HREM Dataset

About

This dataset is an embryonic mouse heart, that has been prepared using the High-Resolution Episcopic Microscopy (HREM) protocol, that involves successively sectioning and imaging of the sample in embedded resin.

The dataset is a 2D image stack generated with a Optical HREM system, it has been downsized (~10%), inverted and brightness adapted for importing into a 3D software with ease.

How to use

The stack can be used simply by importing into most common 3D viewer applications such as Dragonfly, Fiji etc.

If you do not currently have a 3D dataset you can use the 3D viewer application we offer that should allow for simple viewing. The stack has a spacing of X/Y:6um Z: 3um.

Using Indigo 3D Viewer to visualise mouse heart dataset

1. Download our 3D viewer application from our website and the dataset

2. Select 'Volume' in the what do you want to open dialog

3. 

   Open the image stack by double clicking the directory and set the X/Y/Z resolution as required

4. Click 'Render'

5. Now the 3D should appear, use the colour and opacity settings to visualise the model