High-Resolution Episcopic Microscopy produces 3D datasets from detailed 2D sections. This 3D microscopy technique gives volumetric datasets on a wide range of samples.
Image 1-6 micron complete voxel dimensions.
Capable of capturing dense tissue and bone at high contrast.
No sample clearing, optical sectioning or interpolation.
Complete 3D imaging system inclusive of specific software, instrument and other hardware.
What is High Resolution Episcopic Microscopy (HREM)?
High Resolution Episcopic Microscopy (HREM) is a 3D imaging technique for visualising samples ranging from 1-30mm. Resin mounted samples are stained, then sectioned repeatedly with the remaining block face imaged, creating high-resolution stacks at 1–8-micron voxel resolution.
Advantages of High Resolution Episcopic Microscopy (HREM)
HREM gives vast flexibility for a range of samples and tissue density (including bone). Ranging up to 30mm with a minimum of 1mm.
HREM Provides fantastic resolution with as low as 1 micron section thickness and XY scanning.
HREM allows for an easy image processing pipeline with straight forward image outputs. With custom acquire software tailored specifically to the application.
HREM is adaptable, allowing for versatile imaging of various biological samples with exceptional detail and flexibility. From small samples to large samples. No clearing required for any density.
Flexibility for a Range of Samples
High-Resolution Episcopic Microscopy (HREM) delivers exceptional clarity and resolution, even when imaging denser tissues or tissues with varying opacities.
Unlike conventional techniques, High Resolution Episcopic Microscopy (HREM) eliminates the need for tissue clearing, making the imaging process more efficient and convenient. HREM produces high-contrast results, enhancing the visualization of anatomical structures and details.
Capable of visualising samples up to 30mm
Suitable for bone and denser tissue samples
High contrast images
High Resolution for All Tissue Types
High-Resolution Episcopic Microscopy (HREM) offers a variety of resolutions with scanning stages and a choice of optics. It can capture single shots or high-resolution scans at 100x magnification with blending, no blocking.
With sections as thin as 1 µm, from our speciality section system, HREM achieves voxel resolutions of 1-8 µm³, enabling the identification of fine details like individual nerves and blood vessels, surpassing other techniques.
1-8 micron voxel size
XY scanning stage integrated into the software available for larger samples
HREM Easy Data
High Resolution Episcopic Microscopy (HREM) data is readily applicable for both 2D and 3D analysis. Simple software packages like Fiji and Dragonfly can be used to generate impressive images by inverting the LUT (Lookup Table).
High-Resolution Episcopic Microscopy (HREM) images can be manually segmented, owing to their user-friendly 2-Dimensional format, enabling precise measurement of specific features.
Data is stored as image files for easy manipulation
Stored image sizes for easy workflow
No custom image analysis software required
Applications for HREM
High Resolution Episcopic Microscopy (HREM) is used in the study of mouse organs (such as brains and hearts) whole mouse embryos or even plants. As the field of HREM continues to expand, new research papers emerge each year, showcasing the potential in different applications.
Integrate High-Resolution Episcopic Microscopy (HREM) into your research, gaining a valuable tool in your workflow. Explore the accomplishments of our satisfied HREM customers by delving into their published papers.