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Optical High Resolution Episcopic Microscopy(HREM) System

High Resolution Episcopic Microscopy (HREM) is a 3D biomedical imaging technique comprising of 2D sections for structural analysis of samples.

Benefits of the HREM imaging technique:

  • Produces 1-8 micron complete voxel resolution over an entire volumetric dataset

  • Image dense tissue and bone at high contrast without variation

  • No sample clearing, sample clearing or interpolation

  • Capable of imaging up to 60mm samples

Advantages

High Resolution Episcopic Microscopy (HREM) as a 3D imaging technique of ex-vivo samples gives multiple benefits over other techniques such as resolution, cost and more.

This 2D image shows a detailed cross-section of a mouse brain, scanned using High Resolution Episcopic Microscopy (HREM) in the XY plane. Provides high-resolution insights crucial for neurological research and structural analysis.

Flexibility

Image a wide range of samples and density’s with Optical HREM instruments. Image varying density’s and tissue types, including plant matter, with surface based imaging.

This 3D model of a zebrafish was created using High Resolution Episcopic Microscopy (HREM), revealing the intricate internal structures with exceptional detail. The image highlights the zebrafish's anatomy, making it an invaluable tool for developmental biology.

Easy Data

Optical HREM provides straight forward images as an output with little processing. Captured in 2D stacks easily converted into 3D volume renderings.

This 3D model of a mouse, cropped to highlight the heart system, was created using High Resolution Episcopic Microscopy (HREM). The image provides a highly detailed view of the heart’s structure and surrounding anatomy, showcasing the advanced resolution of HREM for precise cardiovascular imaging and research.

Resolution

Output of 1-8 micron voxel resolution gives the flexibility to visualise detail unseen in other techniques and systems. 

High-resolution scan displays a detailed 2D cross-section of a mouse, captured with advanced imaging technology. The image provides clear, precise visualization of the mouse’s anatomical features, essential for in-depth analysis and research in various biological and medical studies.

Adaptable

High-resolution episcopic microscopy (HREM) as a technique requires no sample clearing or interpolation. This opens up the floor to 3D imaging samples in detail.

What is High-Resolution Episcopic Microscopy(HREM)?

High-Resolution Episcopic Microscopy (HREM) is a 3D imaging technique for visualising samples ranging from 1-30mm. Resin mounted samples are stained, then sectioned repeatedly with the remaining block face imaged, creating high-resolution stacks at 1–8-micron voxel resolution.

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We offer two systems for performing HREM, the Micro and Ultra. As well as other products.

Embryonic mouse heart imaged using optical hrem block face imaging system.

Flexibility for a Range of Samples

High-Resolution Episcopic Microscopy(HREM) delivers exceptional clarity and resolution, even when imaging denser tissues or tissues with varying opacities.

 

Unlike conventional techniques, eliminate the need for tissue clearing and interpolation, making the imaging process more efficient and convenient. HREM produces high-contrast results, enhancing the visualization of anatomical structures and details.

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  • Capable of visualising samples up to 30mm in one capture or greater with scanning

  • Suitable for bone and denser tissue samples

  • High contrast images even in higher density areas

This image showcases the detailed 3D morphology of agricultural samples, such as barley, captured using High Resolution Episcopic Microscopy (HREM). HREM provides precise, high-resolution imaging of plant structures, enabling in-depth analysis of cellular and tissue-level morphology critical for agricultural research and crop development.

High Resolution for All Tissue and Sample Types

This 2D image displays a section of a mouse embryo captured using High Resolution Episcopic Microscopy (HREM). A zoomed-in portion highlights individual cells, showcasing the high level of detail and clarity provided by HREM for cellular-level analysis in developmental biology.

Biomedical imaging techniques have evolved significantly, with HREM standing out for its ability to deliver precise 3D tissue images. High-Resolution Episcopic Microscopy (HREM) offers a variety of resolutions with scanning stages and a choice of optics. It can capture single shots or high-resolution scans at 100x magnification with blending, no blocking.

 

With sections as thin as 1 µm, from our speciality section system, HREM achieves voxel resolutions of 1-8 µm³, enabling the identification of fine details like individual nerves and blood vessels, surpassing other techniques.

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  • 1-8 micron voxel size resolution

  • 100x magnification

  • XY scanning stage integrated into the software available for larger samples

Easy Data with HREM

This image features a 2D section of a mouse embryo alongside a 3D reconstruction, both captured using High Resolution Episcopic Microscopy (HREM).

The use of 3D imaging in medical research is growing rapidly, with HREM providing accurate and detailed views of tissue samples. High-Resolution Episcopic Microscopy (HREM) data is readily applicable for both 2D and 3D analysis. Simple software packages like Fiji and Dragonfly can be used to generate impressive images by inverting the LUT (Lookup Table). 

 

HREM images can be manually or automatically segmented in the user-friendly 2-D image format, enabling precise measurements.

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  • Data is stored as image files for easy manipulation (jpeg,tif and more)

  • Stored image sizes for easy workflow

  • No custom image analysis software required

Applications for HREM

High Resolution Episcopic Microscopy(HREM) is used in the study of:

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As the field of HREM continues to expand, new research papers emerge each year, showcasing the potential in different applications.

 

Integrate into your research, gaining a valuable tool in your workflow. Explore the accomplishments of our satisfied HREM customers by delving into their published papers.

This high-resolution image captures the intricate anatomical structure of a mouse heart using High Resolution Episcopic Microscopy (HREM). The clear visualization of the heart’s chambers, vessels, and tissue architecture provides detailed insights into its morphology, making it a valuable tool for studying cardiovascular development and function.

Testimonials

"HREM data has allowed the DMDD team to identify nearly 400 different phenotypes in knockout mouse embryos. These range from large-scale organ malformations to the abnormal development of nerves and blood vessels."

Tim Mohan
(DMDD)

Product Options

View product options for HREM 3D imaging system such as:

  • Micro Optical HREM system for lower budget high quality 3Ds up to 25mm.

  • Ultra Optical HREM systems designed for a range of images

  • Consumables for HREM

HREM Micro Block Face Imaging System

Contact our High-Resolution Episcopic Microscopy (HREM) Experts

Want to know more about HREM, ask for a quote or get questions answered. Contact us and we can help answer all your questions.

Email:
hello@indigo-scientific.co.uk

Thank You!

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